Direct photometric analysis of serum calcium with glyoxal bis (2-hydroxyanil).
نویسندگان
چکیده
A spectrophotometric method for the direct determination of microgram quantities of calcium in serum is presented. In this procedure, 0.02 ml. of serum is interacted with buffered glyoxal bis (2-hydroxyanil) solution (GBHA) at pH 12.6. The resultant red calcium-GBHA complex is diluted in methanol-acetone-water solution, and the absorbance determined at 520 m . The effects of pH, temperature, CBHA concentration, and alcohol-water ratio were evaluated, as was interference by serum constituents. The method is simple and precise, and the analytical results compare well with the chloranilate procedure for serum calcium.
منابع مشابه
Determination of serum calcium by glyoxal bis (2-hydroxyanil) chelation.
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ورودعنوان ژورنال:
- Clinical chemistry
دوره 12 4 شماره
صفحات -
تاریخ انتشار 1966